Use of scintillometric quantitation of unscheduled DNA synthesis in isolated rat hepatocytes for the screening of genotoxic agents
The induction of unscheduled DNA synthesis has been considered as a suitable endpoint for the screening of genotoxic agents. Experimentally, unscheduled DNA synthesis is most frequently measured by autoradiography. The purpose of this report was to examine the usefulness of the liquid scintillation counting technique in measuring unscheduled DNA synthesis response in isolated rat hepatocytes. The various liquid scintillation counting-based unscheduled DNA synthesis assay procedures were examined according to the following groupings: (1) procedures based on the acid precipitation of cellular macromolecules, (2) procedures based on isopycnic gradient centrifugation of solubilized cells, (3) procedures based on nuclei isolation in conjunction with other DNA purification methods, and (4) procedures based on the selective retention of hepatocellular DNA. Limited cases in which test chemicals gave positive unscheduled DNA synthesis response in liquid scintillation counting-based assays and negative unscheduled DNA synthesis response in autoradiography-based assays are presented. It is concluded that liquid scintillation counting-based unscheduled DNA synthesis assays represent an appropriate system for inclusion in carcinogenicity and mutagenicity testing programs.
- Research Organization:
- Univ. of Wisconsin, Madison (USA)
- OSTI ID:
- 5985124
- Journal Information:
- Cell Biol. Toxicol.; (United States), Journal Name: Cell Biol. Toxicol.; (United States) Vol. 3:2; ISSN CBTOE
- Country of Publication:
- United States
- Language:
- English
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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CENTRIFUGATION
COUNTING TECHNIQUES
DNA
DNA REPAIR
DNA REPLICATION
LIVER CELLS
MAMMALS
MUTAGEN SCREENING
MUTAGENS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RATS
RECOVERY
REPAIR
RETENTION
RODENTS
SCINTILLATION COUNTING
SCREENING
SEPARATION PROCESSES
SOMATIC CELLS
ULTRACENTRIFUGATION
VERTEBRATES
XENOBIOTICS