Exposure of mammalian cells to 60-Hz magnetic or electric fields: Analysis of DNA repair of induced, single-strand breaks
- Pacific Northwest Laboratory, Richland, WA (USA)
DNA damage was induced in isolated human peripheral lymphocytes by exposure at 5 Gy to 60Co radiation. Cells were permitted to repair the DNA damage while exposed to 60-Hz fields or while sham-exposed. Exposed cells were subjected to magnetic (B) or electric (E) fields, alone or in combination, throughout their allotted repair time. Repair was stopped at specific times, and the cells were immediately lysed and then analyzed for the presence of DNA single-strand breaks (SSB) by the alkaline-elution technique. Fifty to 75 percent of the induced SSB were repaired 20 min after exposure, and most of the remaining damage was repaired after 180 min. Cells were exposed to a 60-Hz ac B field of 1 mT; an E field of 1 or 20 V/m; or combined E and B fields of 0.2 V/m and 0.05 mT, 6 V/m and 0.6 mT, or 20 V/m and 1 mT. None of the exposures was observed to affect significantly the repair of DNA SSB.
- OSTI ID:
- 5984451
- Journal Information:
- Bioelectromagnetics (New York); (USA), Journal Name: Bioelectromagnetics (New York); (USA) Vol. 11:3; ISSN BLCTD; ISSN 0197-8462
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
560400 -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DNA REPAIR
ELECTROMAGNETIC FIELDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
PRIMATES
RADIOINDUCTION
RADIOISOTOPES
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
YEARS LIVING RADIOISOT