Exposure of mammalian cells to 60-Hz magnetic or electric fields: analysis for DNA single-strand breaks
Chinese hamster ovary (CHO) cells were exposed for 1 h to 60-Hz magnetic fields (0.1 or 2 mT), electric fields (1 or 38 V/m), or to combined magnetic and electric fields (2 mT and 38 V/m, respectively). Following exposure, the cells were lysed, and the DNA was analyzed for the presence of single-strand breaks (SSB), using the alkaline elution technique. No significant differences in numbers of DNA SSB were detected between exposed and sham-exposed cells. A positive control exposed to X-irradiation sustained SSB with a dose-related frequency. Cells exposed to nitrogen mustard (a known cross-linking agent) and X-irradiation demonstrated that the assay could detect cross-linked DNA under our conditions of electric and magnetic field exposures.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- OSTI ID:
- 6578309
- Journal Information:
- Bioelectromagnetics (N.Y.); (United States), Journal Name: Bioelectromagnetics (N.Y.); (United States) Vol. 9:3; ISSN BLCTD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHO CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC FIELDS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
STRAND BREAKS
X RADIATION