Microbial control of hydrogen sulfide production in a porous medium
- Univ. of Oklahoma, Norman, OK (United States)
- Univ. of Tulsa, OK (United States)
The ability of a sulfide- and glutaraldehyde-tolerant strain of Thiobacillus denitrificans (strain F) to control sulfide production in an experimental system of cores and formation water from the Redfield, Iowa natural gas storage facility was investigated. A stable, sulfide-producing biofilm was established in two separate core systems, one of which was inoculated with strain F, and the other core system (control) was treated in an identical manner, but was not inoculated with strain F. When formation water with 10 mM acetate and 5mM nitrate was injected into both core systems, the effluent sulfide concentrations in the control core system ranged from 200-460 {mu}M. In the test core system inoculated with strain F, the effluent sulfide concentrations were lower, ranging from 70-110 {mu}M. In order to determine whether strain F could control sulfide production under optimal conditions for sulfate-reducing bacteria, the electron donor was changed to lactate, and inorganic nutrients (nitrogen and phosphate sources) were added to the formation water. When nutrient-supplemented formation water with 3.1 mM lactate and 10 mM nitrate was used, the effluent sulfide concentrations of the control core system initially increased to about 3800 pM, and then decreased to about 1100 {mu}M after 5 wk. However, in the test core system inoculated with strain F, the effluent sulfide concentrations were much lower, 160-330 {mu}M. Nitrate consumption (5 mM) and high concentrations (101-1011 cells/mL) of strain F were detected in the test core system. An accumulation of biomass occurred in the influent lines during 2 mo of continuous operation, but only a small increase in injection pressure was observed. These studies showed that inoculation with strain F was needed for effective control of sulfide production, and that significant plugging or loss of injectivity owing to microbial inoculation did not occur. 7 refs., 3 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 598247
- Report Number(s):
- CONF-950587-; ISSN 0273-2289; TRN: 98:001503-0027
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 57-58; Conference: 17. symposium on biotechnology for fuels and chemicals, Vail, CO (United States), 7-11 May 1995; Other Information: PBD: Spr 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
09 BIOMASS FUELS
BACTERIA
BIOMASS
CONTROL
NATURAL GAS
NUTRIENTS
PRODUCTION
SULFATE-REDUCING BACTERIA
BIOCONVERSION
BIODEGRADATION
HYDROGEN SULFIDES
STORAGE FACILITIES
INTERSTITIAL WATER
STRAINS
INOCULATION
BACILLUS
NITRATES
RELIABILITY
PERFORMANCE