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Title: Microbial control of hydrogen sulfide production

Abstract

A sulfide-resistant strain of Thiobacillus denitrificans, strain F, prevented the accumulation of sulfide by Desulfovibrio desulfuricans when both organisms were grown in liquid medium. The wild-type strain of T. denitrificans did not prevent the accumulation of sulfide produced by D. desulfuricans. Strain F also prevented the accumulation of sulfide by a mixed population of sulfate-reducing bacteria enriched from an oil field brine. Fermentation balances showed that strain F stoichiometrically oxidized the sulfide produced by D. desulfuricans and the oil field brine enrichment to sulfate. The ability of a strain F to control sulfide production in an experimental system of cores and formation water from the Redfield, Iowa, natural gas storage facility was also investigated. A stable, sulfide-producing biofilm was established in two separate core systems, one of which was inoculated with strain F while the other core system (control) was treated in an identical manner, but was not inoculated with strain F. When formation water with 10 mM acetate and 5 mM nitrate was injected into both core systems, the effluent sulfide concentrations in the control core system ranged from 200 to 460 {mu}M. In the test core system inoculated with strain F, the effluent sulfide concentrations were lower, rangingmore » from 70 to 110 {mu}M. In order to determine whether strain F could control sulfide production under optimal conditions for sulfate-reducing bacteria, the electron donor was changed to lactate and inorganic nutrients (nitrogen and phosphate sources) were added to the formation water. When nutrient-supplemented formation water with 3.1 mM lactate and 10 mM nitrate was used, the effluent sulfide concentrations of the control core system initially increased to about 3,800 {mu}M, and then decreased to about 1,100 {mu}M after 5 weeks. However, in the test core system inoculated with strain F, the effluent sulfide concentrations were much lower, 160 to 330 {mu}M.« less

Authors:
; ; ;  [1]
  1. Univ. of Oklahoma, Tulsa, OK (United States) [and others
Publication Date:
Research Org.:
BDM Oklahoma, Inc., Bartlesville, OK (United States)
OSTI Identifier:
270489
Report Number(s):
CONF-9509173-
ON: DE96001220; TRN: 96:002616-0017
Resource Type:
Conference
Resource Relation:
Conference: International conference on microbial enhanced oil recovery and related biotechnology for solving environment problems, Dallas, TX (United States), 11-14 Sep 1995; Other Information: PBD: [1995]; Related Information: Is Part Of The fifth international conference on microbial enhanced oil recovery and related biotechnology for solving environmental problems: 1995 Conference proceedings; Bryant, R. [ed.]; PB: 619 p.
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; PETROLEUM; PRODUCTION; OIL WELLS; HYDROGEN SULFIDES; REMOVAL; BRINES; DESULFURIZATION; MICROBIAL EOR; BACTERIA; SOUR CRUDES; OIL FIELDS

Citation Formats

Montgomery, A.D., Bhupathiraju, V.K., Wofford, N., and McInerney, M.J.. Microbial control of hydrogen sulfide production. United States: N. p., 1995. Web.
Montgomery, A.D., Bhupathiraju, V.K., Wofford, N., & McInerney, M.J.. Microbial control of hydrogen sulfide production. United States.
Montgomery, A.D., Bhupathiraju, V.K., Wofford, N., and McInerney, M.J.. Sun . "Microbial control of hydrogen sulfide production". United States. https://www.osti.gov/servlets/purl/270489.
@article{osti_270489,
title = {Microbial control of hydrogen sulfide production},
author = {Montgomery, A.D. and Bhupathiraju, V.K. and Wofford, N. and McInerney, M.J.},
abstractNote = {A sulfide-resistant strain of Thiobacillus denitrificans, strain F, prevented the accumulation of sulfide by Desulfovibrio desulfuricans when both organisms were grown in liquid medium. The wild-type strain of T. denitrificans did not prevent the accumulation of sulfide produced by D. desulfuricans. Strain F also prevented the accumulation of sulfide by a mixed population of sulfate-reducing bacteria enriched from an oil field brine. Fermentation balances showed that strain F stoichiometrically oxidized the sulfide produced by D. desulfuricans and the oil field brine enrichment to sulfate. The ability of a strain F to control sulfide production in an experimental system of cores and formation water from the Redfield, Iowa, natural gas storage facility was also investigated. A stable, sulfide-producing biofilm was established in two separate core systems, one of which was inoculated with strain F while the other core system (control) was treated in an identical manner, but was not inoculated with strain F. When formation water with 10 mM acetate and 5 mM nitrate was injected into both core systems, the effluent sulfide concentrations in the control core system ranged from 200 to 460 {mu}M. In the test core system inoculated with strain F, the effluent sulfide concentrations were lower, ranging from 70 to 110 {mu}M. In order to determine whether strain F could control sulfide production under optimal conditions for sulfate-reducing bacteria, the electron donor was changed to lactate and inorganic nutrients (nitrogen and phosphate sources) were added to the formation water. When nutrient-supplemented formation water with 3.1 mM lactate and 10 mM nitrate was used, the effluent sulfide concentrations of the control core system initially increased to about 3,800 {mu}M, and then decreased to about 1,100 {mu}M after 5 weeks. However, in the test core system inoculated with strain F, the effluent sulfide concentrations were much lower, 160 to 330 {mu}M.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

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