Microbial control of biogenic sulfide production
- and others
A sulfide-resistant strain of Thiobacillus denitrificans, strain F, prevented the accumulation of sulfide by Desulfovibrio desulfuricans when both organisms were grown in liquid medium. The wild-type strain of T. denitrificans did not prevent the accumulation of sulfide produced by D. Desulfuricans. Strain F also prevented the accumulation of sulfide by a mixed population of sulfate-reducing bacteria enriched from an oil field brine. Fermentation balances showed that strain F stoichiometrically oxidized the sulfide produced by D. desuotricans and the oil field brine enrichment to sulfate. The ability of a strain F to control sulfide production in an experimental system of cores and formation water from the Redfield, Iowa natural gas storage facility was also investigated. A stable, sulfide-producing biofilm was established in two separate core systems, one of which was inoculated with strain F while the other core system (control) was treated in an identical manner, but was not inoculated with strain F. When formation water with 10 mM acetate and 5 mM nitrate was injected into both core systems, the effluent sulfide concentrations in the control core system ranged from 200 to 460 {mu}M. In the test core system inoculated with strain F, the effluent sulfide concentrations were lower, ranging from 70 to 110 {mu}M. In order to determine whether strain F could control sulfide production under optimal conditions for sulfate-reducing bacteria, the electron donor was changed to lactate and inorganic nutrients (nitrogen and phosphate sources) were added to the formation water. When nutrient-supplemented formation water with 3.1 mM lactate and 10 mM nitrate was used, the effluent sulfide concentrations of the control core system initially increased to about 3800 {mu}M, and then decreased to about 1100 {mu}M after 5 weeks. However, in the test core system inoculated with strain F, the effluent sulfide concentrations were much lower, 160 to 330 {mu}M.
- Research Organization:
- US Department of Energy (USDOE), Washington DC (United States); Amoco Production Co., Houston, TX (United States); Conoco, Inc., Stamford, CT (United States)
- OSTI ID:
- 377198
- Report Number(s):
- CONF-9509296-; ON: DE96001221; TRN: 96:004054-0033
- Resource Relation:
- Conference: 2. international petroleum environmental conference: environmental issues and solutions in petroleum exploration, production, and refining, New Orleans, LA (United States), 25-27 Sep 1995; Other Information: PBD: 1995; Related Information: Is Part Of Environmental issues and solutions in petroleum exploration, production and refining; Sublette, K.L. [ed.]; PB: 1078 p.
- Country of Publication:
- United States
- Language:
- English
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