Production of cellulase systems by selected mutants of Trichoderma reesei in soild-state fermentation and their hydrolytic potentials

Journal Article · Tue Dec 31 04:00:00 EST 1996 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02941726· OSTI ID:598221

Awafo, V A ^[1]; Chahal, D S; Simpson, B K ^[2]; Le, G B.B. ^[3]

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1. Universite du Quebec, Laval (Canada)
2. McGill Univ., Quebec (Canada)
3. Ministry of Natural Resources, Quebec (Canada)

Three mutants of Trichoderma reesei were grown in solid-state fermentation (SSF) in flasks and in a pan bioreactor. Mutant strain MCG 80 proved to be the best at producing an optimal cellulose system using lignocellulosic material (wheat straw [WS]) as substrate. This preparation exhibited a {beta}-glucosidase activity (PGA) to FPA (FPA) ratio of about 1.0, which is indicative of a high potential for hydrolysis of cellulose. The yields of cellulose systems and the ratio of PGA to FPA produced in flasks were comparable to that of the pan bioreactor. The cellulose system of T. reesei MCG 80 having a ratio of {beta}GA to FPA close to 1.0 gave the most complete (88-95%) hydrolysis of 5% delignified wheat straw (DWS). On the other hand, the cellulose system of cocultures of T. reesei QMY-1 and Aspergillus phoenicis failed to produce high hydrolytic yields in spite of having a very high ratio of PGA to FPA (3.04). This failure was owing to the fact that coculture contained the relatively poor-quality cellulase system of the dominant organism, A. phoenicis. The resulting fermented WS can be used, as a source of enzyme (unextracted), for hydrolysis of wheat straw, and it gives increased yields of reducing sugars compared to analogous extracted enzyme preparations. The hydrolytic potential of two commercial enzymes tested were considerably lower than those of the cellulose systems produced on WS. It is evident that a complete cellulose system having a {beta}GA-to-FPA ratio close to 1.0 and high hydrolytic potential can be produced on lignocellulosic feedstocks in SSF. 21 refs., 3 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 598221
• Report Number(s): CONF-950587--
• Journal Information: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Vol. 57-58; ISSN ABIBDL; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ASPERGILLUS
BIOCONVERSION
BIOREACTORS
CELLULASE
CELLULOSE
CHEMICAL FEEDSTOCKS
CHEMICAL REACTION YIELD
ENZYMATIC HYDROLYSIS
ENZYMES
FERMENTATION
GLUCOSIDASE
MICROORGANISMS
MUTANTS
PRODUCTION
RENEWABLE ENERGY SOURCES
SUBSTRATES
TRICHODERMA