Combined submerged and solid substrate fermentation for the bioconversion of lignocellulose

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260300219· OSTI ID:5692730

Viesturs, U E; Strikauska, S V; Leite, M P; Berzins, A J; Tengerdy, R P

A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bio-convertibility. SSF was conducted in the top part of the bioreactor by inoculating the straw with a 36-h mycelial culture of T. reesei, or Coriolus versicolor. In the bottom part of the fermenter, Endomycopsis fibuliger was grown in SF. The SF liquor was recirculated through the SSF stage at 24 hour intervals to remove glucose and other metabolites that may inhibit growth, and to maintain optimum moisture level and temperature. The removed glucose and other metabolites provided nutrients for the yeast in the SF stage. The combined fermentation resulted in overall higher biomass yield, increased bioconversion, increased cellulase production, and increased digestibility compared with single SSF or SF. (Refs. 16).

• Research Organization: Latvian Academy of Sciences, 226067 Riga, Kleisti, Latvian SSR, USSR. August Kirchenstein Institute of Microbiology; Colorado State Univ., Fort Collins, CO, 80523
• OSTI ID: 5692730
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 30:2; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ADSORPTION
ALDEHYDES
BIOCONVERSION
BIOMASS
BIOREACTORS
BODY
CARBOHYDRATES
CELLULASE
CELLULOSE
CEREALS
CONTROL
CULTURE MEDIA
DESIGN
ENERGY SOURCES
ENZYMES
FERMENTATION
FUNGI
GLUCOSE
GLYCOSYL HYDROLASES
GRASS
HEXOSES
HYDROLASES
INHIBITION
INOCULATION
LIGNIN
METABOLITES
MOISTURE
MONOSACCHARIDES
MYCELIUM
NUTRIENTS
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
PLANT TISSUES
PLANTS
POLYSACCHARIDES
RECYCLING
REMOVAL
RENEWABLE ENERGY SOURCES
SACCHARIDES
SORPTION
STEAM
STRAW
SUBSTRATES
TEMPERATURE CONTROL
TISSUES
TRICHODERMA
TRICHODERMA VIRIDE
WHEAT
YIELDS