Production of cellulase in solid-state fermentation with Trichoderma reesei MCG 80 on wheat straw

Journal Article · Tue Dec 31 04:00:00 EST 1996 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02941724· OSTI ID:598217

Chahal, P S; Chahal, D S ^[1]; Le, G B.B. ^[2]

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1. Universite du Quebec, Laval (Canada)
2. Ministry of Natural Resources, Charlesbourg, Quebec (Canada)

It is an accepted fact that ethanol production from lignocellulosic materials is not economical as yet because of the high cost of cellulose production. To reduce the cost of cellulose production, lignocellulosic material (wheat straw [WS]), a comparatively much cheaper substrate, was used instead of costly substrates (pure cellulose or lactose). A pan bioreactor was developed for solid-state fermentation (SSF) that required a small capital investment. High yields of complete cellulose system were obtained compared to that in the liquid-state fermentation (LSF) from WS, when treated with 4.25% NaOH at 121{degrees}C for 1 h and mixed with Mandels` medium. A complete cellulose system is defined as one in which the ratio of {beta} glucosidase activity to filter paper activity in the enzyme solution is close to 1.0. The cellulose system derived from SSF using the pan bioreactor gave more than 85% hydrolysis of delignified WS. The prototype pan bioreactor requires further improvements so that optimum quantity of substrate can be fermented to obtain high yields of complete cellulose system per unit space. The SSF process provides a means for the production of complete cellulose system for the economical bioconversion of renewable biomass into ethanol. 18 refs., 5 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 598217
• Report Number(s): CONF-950587--
• Journal Information: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Vol. 57-58; ISSN ABIBDL; ISSN 0273-2289
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOCONVERSION
BIOMASS
BIOREACTORS
CAPITAL
CELLULASE
CELLULOSE
CHEMICAL REACTION YIELD
COST
DESIGN
ENZYMATIC HYDROLYSIS
ETHANOL
FERMENTATION
GLUCOSIDASE
HYDROLYSIS
INVESTMENT
LACTOSE
OPTIMIZATION
PAPER
PRODUCTION
RENEWABLE ENERGY SOURCES
STRAW
SUBSTRATES
TRICHODERMA