Following macromolecular interactions and sugar metabolism using site specific /sup 3/H labelling and NMR spectroscopy
Conference
·
OSTI ID:5982024
In this paper we discuss the application of /sup 3/H NMR to biological problems. Two specific examples will be described; first, analysis of the binding of maltose to its transport protein from E. coli, called MBP; and second, following the glycolytic metabolism of glucose in erythrocytes. In both of these cases the unique properties of /sup 3/H for magnetic resonance make possible observations which are difficult with other methods. 4 refs., 2 figs.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 5982024
- Report Number(s):
- LBL-27196; CONF-880787-6; ON: DE89013689
- Resource Relation:
- Conference: 3. international symposium on the synthesis and applications of isotopically labeled compounds, Innsbruck, Austria, 17 Jul 1988; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOLOGICAL PATHWAYS
EVALUATION
GLUCOSE
BIOCHEMICAL REACTION KINETICS
MALTOSE
ERYTHROCYTES
ESCHERICHIA COLI
LABELLING
METABOLISM
NMR SPECTRA
TRITIUM
ALDEHYDES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
DISACCHARIDES
HEXOSES
HYDROGEN ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SPECTRA
YEARS LIVING RADIOISOTOPES
550501* - Metabolism- Tracer Techniques
400102 - Chemical & Spectral Procedures
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BIOLOGICAL PATHWAYS
EVALUATION
GLUCOSE
BIOCHEMICAL REACTION KINETICS
MALTOSE
ERYTHROCYTES
ESCHERICHIA COLI
LABELLING
METABOLISM
NMR SPECTRA
TRITIUM
ALDEHYDES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
DISACCHARIDES
HEXOSES
HYDROGEN ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SPECTRA
YEARS LIVING RADIOISOTOPES
550501* - Metabolism- Tracer Techniques
400102 - Chemical & Spectral Procedures