Effect of storage media on the proliferation of periodontal ligament fibroblasts
The effect of storage media, which are routinely used in replantation, upon the proliferative capacity of periodontal ligament fibroblasts, was compared with the effect of a tissue culture medium. The periodontal tissue was obtained from mandibular central incisors of White New Zealand rabbits. The experiments were performed in fibroblasts derived during second subculture. The storage media were physiologic salt solution, Ringer's solution and Rivanol; the tissue culture medium was alpha-minimum essential medium without nucleosides. The incubation period was 1 hour. (/sup 3/H)-thymidine incorporation and cell counts were taken to indicate changes in the proliferative capacity of the fibroblasts. The tissue culture experiments showed that the proliferative ability of the periodontal ligament fibroblasts was dependent upon the composition of the storage medium. Physiologic salt solution, Ringer's solution and Rivanol were unable to maintain the metabolism of the fibroblasts. alpha-MEM medium, however, was capable of stimulating proliferation of the periodontal ligament fibroblasts.
- Research Organization:
- Poliklinik fuer Zahnaerztliche Prothetik, Muenchen, Germany, F.R.
- OSTI ID:
- 5977732
- Journal Information:
- J. Periodontol.; (United States), Vol. 58:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CULTURE MEDIA
CHEMICAL COMPOSITION
FIBROBLASTS
CELL PROLIFERATION
LIGAMENTS
IMPLANTS
RABBITS
STORAGE
TEETH
THYMIDINE
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORAL CAVITY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
TISSUES
VERTEBRATES
550301* - Cytology- Tracer Techniques