Effect of storage media on the proliferation of periodontal ligament fibroblasts

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · J. Periodontol.; (United States)

DOI:https://doi.org/10.1902/jop.1987.58.7.481· OSTI ID:5977732

Lauer, H C; Mueller, J G; Gross, J; Horster, M F

The effect of storage media, which are routinely used in replantation, upon the proliferative capacity of periodontal ligament fibroblasts, was compared with the effect of a tissue culture medium. The periodontal tissue was obtained from mandibular central incisors of White New Zealand rabbits. The experiments were performed in fibroblasts derived during second subculture. The storage media were physiologic salt solution, Ringer's solution and Rivanol; the tissue culture medium was alpha-minimum essential medium without nucleosides. The incubation period was 1 hour. (/sup 3/H)-thymidine incorporation and cell counts were taken to indicate changes in the proliferative capacity of the fibroblasts. The tissue culture experiments showed that the proliferative ability of the periodontal ligament fibroblasts was dependent upon the composition of the storage medium. Physiologic salt solution, Ringer's solution and Rivanol were unable to maintain the metabolism of the fibroblasts. alpha-MEM medium, however, was capable of stimulating proliferation of the periodontal ligament fibroblasts.

• Research Organization: Poliklinik fuer Zahnaerztliche Prothetik, Muenchen, Germany, F.R.
• OSTI ID: 5977732
• Journal Information: J. Periodontol.; (United States), Journal Name: J. Periodontol.; (United States) Vol. 58:7; ISSN JOPRA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CELL PROLIFERATION
CHEMICAL COMPOSITION
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DIGESTIVE SYSTEM
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
IMPLANTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGAMENTS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORAL CAVITY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RABBITS
RIBOSIDES
SOMATIC CELLS
STORAGE
TEETH
THYMIDINE
TISSUE CULTURES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES