Identification and quantitation of sulfamethazine metabolites by liquid chromatography and gas chromatography-mass spectroscopy
Methods for the identification and quantitation of carbon-14 labeled sulfamethazine (4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide), N/sup 4/-acetylsulfamethazine, the N/sup 4/-glucose conjugate of sulfamethazine, and desaminosulfamethazine in swine tissue are described. Tissues are ground and extracted with methanol, and the /sup 14/C-labeled compounds are purified by XAD-2 column chromatography and C-18 reverse phase liquid chromatography (LC) and the /sup 14/C-labeled compounds are then methylated and identified by gas chromatography-mass spectrometry analysis. Quantitation is accomplished by measuring the amount of /sup 14/C-activity that cochromatographs (C-18 reverse phase LC) with reference compounds.
- Research Organization:
- Dept. of Agriculture, Fargo, ND
- OSTI ID:
- 5965332
- Journal Information:
- J. Assoc. Off. Anal. Chem.; (United States), Journal Name: J. Assoc. Off. Anal. Chem.; (United States) Vol. 68:5; ISSN JANCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
AMIDES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DISEASES
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
GAS CHROMATOGRAPHY
INFECTIOUS DISEASES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MASS SPECTROSCOPY
METABOLITES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
SULFONAMIDES
SWINE
TISSUE DISTRIBUTION
VERTEBRATES