Variation in radiation sensitivity during the cell cycle of two human squamous cell carcinomas

Journal Article · Mon Apr 01 04:00:00 EST 1991 · International Journal of Radiation Oncology, Biology and Physics; (USA)

DOI:https://doi.org/10.1016/0360-3016(91)90016-W· OSTI ID:5956517

Quiet, C A; Weichselbaum, R R; Grdina, D J ^[1]

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1. Michael Reese Medical Center, Chicago, IL (USA)

Changes in the radiation age response are described in two cell lines derived from human squamous cell carcinomas. A radioresistant tumor cell line, JSQ-3, has a DO of 240 cGy and is polyploid with a DNA content of 2.68. A relatively radiosensitive tumor cell line, SCC-61, has a DO of 126 cGy and has a DNA index of 1.16. Tumor cells were separated and synchronized by centrifugal elutriation; flow cytometry was used to determine cell-cycle parameters and relative synchrony. The radioresistant cell line, JSQ-3B, was found to have twice the number of cells in S-phase than the more sensitive cell line (28% and 13% for JSQ-3B and SCC-61B, respectively). Both cell lines, despite differences in intrinsic radiosensitivity, were most resistant during S-phase (DOs of 258 and 157 cGy for JSQ-3B and SCC-61B, respectively) and were maximally sensitive during G1 (DOs of 193 and 95 cGy for JSQ-3B and SCC-61B, respectively). Clinical implications of our findings are discussed.

• OSTI ID: 5956517
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (USA), Journal Name: International Journal of Radiation Oncology, Biology and Physics; (USA) Vol. 20:4; ISSN IOBPD; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CARCINOMAS
CELL CYCLE
CELL FLOW SYSTEMS
DISEASES
DNA
DOSE-RESPONSE RELATIONSHIPS
MAMMALS
MAN
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIOSENSITIVITY
TUMOR CELLS
VERTEBRATES