9-{beta}-arabinofuranosyladenine preferentially sensitizes radioresistant squamous cell carcinoma cell lines to x-rays
- Rush Univ. Medical Center, Chicago, IL (United States). Therapeutic Radiology
- Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology
The effect of 9-{beta}-arabinofuranosyladenine (ara-A) on sensitivity to the deleterious effects of x-rays was studied in six squamous cell carcinoma cell lines. Three lines were relatively radioresistant, having D{sub 0} values of 2.31 to 2.89 Gy, and the other three lines were relatively radiosensitive, having D{sub 0} values of between 1.07 and 1.45 Gy. Ara-A (50 or 500 {mu}M) was added to cultures 30 min prior to irradiation and removed 30 min after irradiation, and sensitivity was measured in terms of cell survival. The radiosensitizing effect of ara-A was very dependent on the inherent radiosensitivity of the tumor cell line. Fifty micromolar concentrations of ara-A sensitized only the two most radioresistant lines, SCC-12B.2 and JSQ-3. Five hundred micromolar concentrations of ara-A sensitized the more sensitive cell lines, SQ-20B and SQ-9G, but failed to have any effect on the radiation response of the two most sensitive cell lines, SQ-38 and SCC-61. Concentrations of ara-A as low as 10 {mu}M were equally efficient in inhibiting DNA synthesis in all six cell lines. These results suggest that the target for the radiosensitizing effect of ara-A is probably related to the factor controlling the inherent radiosensitivity of human tumor cells. Therefore, ara-A might be useful in overcoming radiation resistance in vivo.
- Research Organization:
- Argonne National Lab., IL (United States); Chicago Univ., IL (United States). Dept. of Radiation and Cellular Oncology
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Cancer Inst., Bethesda, MD (United States)
- DOE Contract Number:
- W-31109-ENG-38; FG02-88ER60661
- OSTI ID:
- 10149645
- Report Number(s):
- ANL/BIM/PP--76541; ON: DE94011510; BR: HA0202010; CNN: CA 42596
- Country of Publication:
- United States
- Language:
- English
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560120
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON 14 COMPOUNDS
CELL CULTURES
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RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
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RADIOSENSITIZERS
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TRACER TECHNIQUES
TRITIUM COMPOUNDS
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON 14 COMPOUNDS
CELL CULTURES
MAN
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
SURVIVAL TIME
TRACER TECHNIQUES
TRITIUM COMPOUNDS
X RADIATION