High resolution scanning electron microscopy of rabbit corneal endothelium to show effects of UV-visible irradiation in the presence of chlorpromazine

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Lens and Eye Toxicity Research; (USA)

OSTI ID:5953213

Lea, P J; Hollenberg, M J; Menon, I A; Temkin, R J; Persad, S D; Basu, P K ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The ultrastructure of rabbit cornea endothelial cells was examined by scanning electron microscopy (SEM) in freeze-cleaved corneas using a Hitachi S-570 scanning electron microscope in the high resolution mode (HRSEM). In order to study phototoxic effects in vitro, rabbit corneas (experimental) were cultured as organ culture in the presence of 5 micrograms/ml chlorpromazine (CPZ) and irradiated. For comparison, control 1 corneas were not irradiated but incubated in the dark without CPZ in the medium; control 2 corneas were also kept in the dark but in the presence of CPZ; control 3 corneas were irradiated with no CPZ in the medium. Cellular damage was not seen in the three types of control corneas, but in the experimental corneas the endothelial cells showed extensive disruption of the cell membrane and some deterioration of the intracellular components. Our study confirmed that HRSEM is a satisfactory new technique for visualizing damage of the intracellular organelles of corneal endothelium.

• OSTI ID: 5953213
• Journal Information: Lens and Eye Toxicity Research; (USA), Journal Name: Lens and Eye Toxicity Research; (USA) Vol. 6:1-2; ISSN 1042-6922; ISSN LETRE
• Country of Publication: United States
• Language: English

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560120* -- Radiation Effects on Biochemicals
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
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BIOLOGICAL EFFECTS
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MAMMALS
MICROSCOPY
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ORGANIC CHLORINE COMPOUNDS
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ORGANIC NITROGEN COMPOUNDS
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ORGANS
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