Toxicity of methods of implant material sterilization on corneal endothelium

Journal Article · Thu Oct 31 23:00:00 EST 1985 · Ann. Ophthalmol.; (United States)

OSTI ID:5878044

Singh, G; Boehnke, Mv; von Domarus, D; Draeger, J

The toxicity of different procedures utilized for the sterilization of intraocular implant material was assessed on the endothelium of organ-cultured porcine corneas. Polymethylmethacrylate lenses sterilized by treatment with sodium hydroxide (NaOH), ethylene oxide, formaldehyde, and gamma radiation were added to a culture medium containing normal porcine corneas. Considering the viability of endothelial cells, appearance of intracellular degenerative vacuoles, and denudation of corneal Descemet's membrane as criterion for the evaluation of toxicity of different methods of sterilization, the NaOH-treated lenses were found to be the least toxic to porcine corneal endothelium. Phase-contrast microscopy and vital staining of the endothelium permitted direct viewing of the endothelium aiding in the assessment of toxicity.

• Research Organization: Universitaets-Augenklinik, Hamburg, Germany, F.R.
• OSTI ID: 5878044
• Journal Information: Ann. Ophthalmol.; (United States), Journal Name: Ann. Ophthalmol.; (United States) Vol. 11; ISSN ANOPB
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
BODY AREAS
COMPARATIVE EVALUATIONS
CORNEA
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
EYES
FACE
FORMALDEHYDE
GAMMA RADIATION
HEAD
HYDROGEN COMPOUNDS
HYDROXIDES
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RADIATIONS
RADIOSTERILIZATION
SENSE ORGANS
SODIUM COMPOUNDS
SODIUM HYDROXIDES
STERILIZATION
SWINE
TISSUES
TOXICITY
TRANSPLANTS
VERTEBRATES