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Distribution of hydrogenase genes in Desulforvibrio spp. and their use in identification of species from the oil field environment

Journal Article · · Applied and Environmental Microbiology; (USA)
OSTI ID:5947708
;  [1];  [2]; ;  [3]
  1. Univ. of Calgary, Alberta (Canada)
  2. Nova Husky Research Corporation, Calgary, Alberta (Canada)
  3. Univ. of Alberta, Edmonton (Canada)
The distribution of genes for (Fe), (NiFe), and (NiFeSe) hydrogenases was determined for 22 Desulfovibrio species. The genes for (NiFe) hydrogenase were present in all species, whereas those for the (Fe) and (NiFeSe) hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio did not react with the (NiFe) hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.
OSTI ID:
5947708
Journal Information:
Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:12; ISSN 0099-2240; ISSN AEMID
Country of Publication:
United States
Language:
English