Inhibition studies of three classes of Desulfovibrio hydrogenase: application to the further characterization of the multiple hydrogenases found in Desulfovibrio vulgaris Hildenborough
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The three types of hydrogenase hitherto characterized in genus Desulfovibrio exhibit distinctive inhibition patterns of their proton-deuterium exchange activity by CO, NO and NO/sub 2/-. The (Fe) and (NiFeSe) hydrogenases are the most sensitive to all three inhibitors while the (NiFe) enzymes, relatively little inhibited by CO, are still very sensitive to NO but unaffected by NO/sub 2/-. These differences together with some specific catalytic properties, in particular the pH profile and the H/sub 2/ to HD ratio in the exchange reaction, constitute a simple means of characterizing multiple hydrogenases present in one or different species.
- Research Organization:
- ARBS, CEN Cadarache, Saint Paul Lez Durance, France
- OSTI ID:
- 6168737
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 146:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Mon Sep 24 00:00:00 EDT 2007
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OSTI ID:932520
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BARYONS
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL PROPERTIES
DESULFOVIBRIO
DEUTERIUM
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HYDROGEN ISOTOPES
HYDROGENASES
INHIBITION
ISOENZYMES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NITRIC OXIDE
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTONS
STABLE ISOTOPES
SULFATE-REDUCING BACTERIA
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BARYONS
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL PROPERTIES
DESULFOVIBRIO
DEUTERIUM
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HYDROGEN ISOTOPES
HYDROGENASES
INHIBITION
ISOENZYMES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NITRIC OXIDE
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTONS
STABLE ISOTOPES
SULFATE-REDUCING BACTERIA