Effects of acetylene on hydrogenases from the sulfate reducing and methanogenic bacteria

Journal Article · Tue May 30 04:00:00 EDT 1989 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(89)91570-2· OSTI ID:5696598

He, S H; Woo, S B; DerVartanian, D V; Le Gall, J; Peck, Jr, H D ^[1]

---

1. Univ. of Georgia, Athens (United States)

The effect of acetylene on the activity of the three types of hydrogenase from the anaerobic sulfate reducing bacteria has been investigated. The (Fe) hydrogenase is resistant to inhibition by acetylene while the nickel-containing hydrogenases are inhibited by acetylene with the (NiFe) hydrogenase being 10-50 fold more sensitive than the (NiFeSe) hydrogenase. In addition the Ni(3) EPR signal (g{approximately}2.3) of the 'as isolated' (NiFe) hydrogenase was significantly decreased in intensity upon exposure to acetylene.

• DOE Contract Number: AS09-79ER10499
• OSTI ID: 5696598
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 161:1; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLENE
ALKYNES
BACTERIA
BIOLOGICAL EFFECTS
ELECTRON SPIN RESONANCE
ENZYME ACTIVITY
ENZYMES
HYDROCARBONS
HYDROGENASES
INHIBITION
MAGNETIC RESONANCE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RESONANCE
SULFATE-REDUCING BACTERIA