Prolactin messenger ribonucleic acid concentrations throughout the ovine estrous cycle: Assessment relative to prolactin serum and pituitary amounts

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90456-L· OSTI ID:5924737

Landefeld, T; Roulia, V; Bagnell, T; Ballard, T; Levitan, I ^[1]

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1. Univ. of Michigan, Ann Arbor (USA)

Prolactin (PRL) mRNA concentrations were assessed by nucleic acid hybridization assays in pituitaries of ewes representing the defined stages of the ovine estrous cycle. Concomitantly, pituitary and serum PRL concentrations were measured in these ewes using radioimmunoassays. It was observed that PRL serum, pituitary and mRNA concentrations tended to increase near the time of the gonadotropin preovulatory surge, particularly between 24 hrs before behavioral estrus to 5 hours after estrus. However, the changes in PRL mRNA, serum and pituitary concentrations were shown not to be statistically significant. These data suggest that PRL production during the sheep estrous cycle is maintained without dramatic changes in synthesis or secretion.

• OSTI ID: 5924737
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:12; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOASSAY
BIOSYNTHESIS
BODY
DIAGNOSTIC TECHNIQUES
DNA HYBRIDIZATION
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ESTROUS CYCLE
GLANDS
GONADOTROPINS
HORMONES
HYBRIDIZATION
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LTH
MAMMALS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RNA
RUMINANTS
SHEEP
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES