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Polyamines in rice seedlings under oxygen-deficit stress

Journal Article · · Plant Physiology; (USA)
DOI:https://doi.org/10.1104/pp.91.3.1197· OSTI ID:5923304
Incubation of 3-d-old seedlings of Oryza sativa L. cv Arborio under anaerobic conditions, leads to a large increase in the titer of free putrescine while aerobic incubation causes a slight decrease. After 2 days, the putrescine level is about 2.5 times greater without oxygen than in air. The rice coleoptile also accumulates a large amount of bound putrescine and, to a lesser extent, spermidine and spermine (mainly as acid-soluble conjugates). Accumulation of conjugates in the roots is severely inhibited by the anaerobic treatment. Feeding experiments with labeled amino acids showed that anoxia stimulates the release of {sup 14}CO{sub 2} from tissues fed with ({sup 14}C)arginine and that arginine is the precursor in putrescine biosynthesis. After 2 d of anoxia, the activity of arginine decarboxylase was 42% and 89% greater in coleoptile and root, respectively, than in the aerobic condition. The causes of the differences in polyamine metabolism in anoxic coleoptiles and roots are discussed.
OSTI ID:
5923304
Journal Information:
Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 91:3; ISSN 0032-0889; ISSN PLPHA
Country of Publication:
United States
Language:
English

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