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Polyamines in lymphocyte activation

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6074150
; ;

New features of polyamine metabolism have been identified in both the acid-soluble and the acid-insoluble fraction of activated murine splenic lymphocytes. Lymphocytes activated by the B cell mitogen LPS and simultaneously exposed to either 3H-putrescine or /sup 3/H-spermidine show a differential level of metabolism of the two radioactive polyamines. The percent uptake of isotope from the medium is the same for either putrescine or spermidine during the 48 hour activation period. Intracellularly, only 12% of the label take up as putrescine remains as putrescine and the rest is primarily metabolized to spermidine (47%) and spermine (22%). By contrast, 69% of the initial radioactive spermidine remains as spermidine, again a similar percentage is metabolized to spermine (22%), and a small amount appears as putrescine (2.5%). In these studies the authors have demonstrated the presence of two new small radioactive conjugates (5-7%). Radioactive spermidine is 3 times more effective than radioactive putrescine as a precursor of label in acid-insoluble material. Acid-insoluble label derived from putrescine represents 1.8% of the total cell-associated radioactivity whereas that derived from spermidine is 6.0%. HPLC molecular sieving reveals three distinct radioactive macromolecules.

Research Organization:
Yale Medical School, New Haven, CT
OSTI ID:
6074150
Report Number(s):
CONF-870644-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOCYTES
MAMMALS
MATERIALS
METABOLISM
MICE
ORGANIC COMPOUNDS
PUTRESCINE
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SPERMIDINE
SPLEEN CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES