Polyamine synthesis in maize cell lines

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.90.4.1378· OSTI ID:6610631

Hiatt, A ^[1]

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1. Scripps Clinic and Research Foundation, La Jolla, CA (USA)

Uptake of ({sup 14}C)putrescine, ({sup 14}C)arginine, and ({sup 14}C)ornithine was measured in five separate callus cell lines of Zea mays. Each precursor was rapidly taken into the intracellular pool in each culture where, on the average 25 to 50% of the total putrescine was found in a conjugated form, detected after acid hydrolysis. Half-maximal labeling of each culture was achieved in less than 1 minute. Within this time frame of precursor incorporation, only putrescine derived from arginine was conjugated, indicating that putrescine pools derived from arginine may initially be sequestered from ornithine-derived putrescine. The decarboxylase activities were measured in each culture after addition of exogenous polyamine to the growth medium to assess differential regulation of the decarboxylases. Arginine and ornithine decarboxylase activities were augmented by added polyamine, the effect on arginine decarboxylase being eightfold greater than on ornithine decarboxylase. Levels of extractable ornithine decarboxylase were consistently 15- to 100-fold higher than arginine decarboxylase, depending on the titer of extracellular polyamine. Taken as whole the results support the idea that there are distinct populations of polyamine that are initially sequestered after the decarboxylase reactions and that give rise to separate end products and possibly have separate functions.

• OSTI ID: 6610631
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 90:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ARGININE
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CEREALS
DECARBOXYLASES
ENZYME ACTIVITY
ENZYMES
GRASS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LILIOPSIDA
LYASES
MAGNOLIOPHYTA
MAIZE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORNITHINE
PLANT CELLS
PLANTS
PRECURSOR
PUTRESCINE
SYNTHESIS
TRACER TECHNIQUES