Loss of nitrous oxide reductase in Pseudomonas aeruginosa cultured under N sub 2 O as determined by rocket immunoelectrophoresis

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:5921876

SooHoo, C K; Hollocher, T C ^[1]

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1. Brandeis Univ., Waltham, MA (USA)

Pseudomonas aeruginosa is unusual among denitrifiers in that it grows poorly on N{sub 2}O alone but can grow efficiently on the N{sub 2}O produced as a metabolic intermediate during the reduction of nitrate or nitrite to N{sub 2}. Previous studies in our laboratory have determined that the immediate cause for poor growth on N{sub 2}O is a progressive loss of nitrous oxide uptake and reductase activities in cells grown under N{sub 2}O. In this study, we determined by using rocket immunoelectrophoresis that the mass ratio of nitrous oxide reductase to total protein in the soluble protein fraction of Pseudomonas aeruginosa P2 was highest in cells grown on nitrate, decreased in cells grown on N{sub 2}O following the exhaustion of the initial charge of nitrate, and was nearly zero in cells exposed solely to N{sub 2}O.

• OSTI ID: 5921876
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:11; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CHALCOGENIDES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
KINETICS
METABOLISM
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PSEUDOMONAS
REACTION KINETICS