Growth of Pseudomonas aeruginosa on nitrous oxide
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:5392823
Three strains of Pseudomonas aeruginosa were grown anaerobically on exogenous N/sub 2/O in a defined medium under conditions that assured the maintenance of highly anaerobic conditions for periods of 1 week or more. The bacteria were observed reproducibly to increase their cell density by factors of 3 to 9, but not more, depending on the initial amount of N/sub 2/O. Growth on N/sub 2/O was cleanly blocked by acetylene. Cell yields, CO/sub 2/ production, and N/sub 2/O uptake all increased with initial P/sub N/sub 2/O/ at P/sub N/sub 2/O/ less than or equal to 0.1 atm. Growth curves were atypical in the sense that growth rates decreased with time. This is the first observation of growth of P. aeruginosa on N/sub 2/O as the sole oxidant. N/sub 2/O was shown to be an obligatory, freely diffusible intermediate during growth of strains PAO1 and P1 on nitrate. All three strains used this endogenous N/sub 2/O efficiently for growth. For strains PAO1 and P1, it was confirmed that exogenous N/sub 2/O had little effect on the cell yields of cultures growing with nitrate. On the other hand, strain P2 grew abundantly on exogenous N/sub 2/O when small and growth-limiting concentrations of nitrate of nitrite (2 to 10 mM) were included in the medium. The phenotype of strain P2 suggests that the ability of strains PAO1 and P1 to grow on endogenous N/sub 2/O may also be under control of nitrate or a metabolic product of nitrate. The metabolic defect that prevents abundant growth of strains PAO1 and P1 on exogenous N/sub 2/O was not traceable to dysfunction of the respiratory proton pump, high proton permeability of the membrane, failure to form or maintain a proton motive force, or a nutritional requirement. The metabolic defect is not understood at present.
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 5392823
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 51:6; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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