Loss of N/sub 2/O reductase activity as an explanation for poor growth of Pseudomonas aeruginosa on N/sub 2/O

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5873792

Snyder, S W; Bazylinski, D A; Hollocher, T C

N/sub 2/O uptake activity of cells and N/sub 2/O reductase activity of the soluble fraction from denitrifying bacteria were assayed. Pseudomonas aeruginosa strains PAO1 and P1 lost most of their N/sub 2/O uptake activity and the ability to grow well on N/sub 2/O within 2 to 5 h after exposure to N/sub 2/O. Extensive loss of N/sub 2/O reductase activity accompanied the nearly complete loss of N/sub 2/O uptake activity under N/sub 2/O. Paracoccus denitrificans retained much, but not all, of both activities and the ability to grow vigorously on N/sub 2/O. The pattern with P. aeruginosa strain P2 resembled that for PAO1 and P1 except that loss of the activities proceeded at a slower rate and growth could continue for up to 12 h after exposure to N/sub 2/O. The inability of a number of P. aeruginosa strains to grow well on N/sub 2/O is therefore a direct consequence of the nearly complete loss of N/sub 2/O reductase activity. Turnover-dependent inactivation of N/sub 2/O reductase and its reactivation under reducing conditions occurred in vitro for the enzyme from P. aeruginosa and Paracoccus denitrificans. These events may be significant in determining the activity level of N/sub 2/O reductase in denitrifying bacteria during N/sub 2/O respiration.

• Research Organization: Brandeis Univ., Waltham, MA
• OSTI ID: 5873792
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:9; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIODEGRADATION
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
GROWTH
METABOLISM
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PSEUDOMONAS