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Title: Capillary zone electrophoresis-mass spectrometry of peptides and proteins

Abstract

Capillary zone electrophoresis (CZE) is attracting extensive attention as a fast, high resolution analytical and micro-preparative separations technique for systems of biological interest. In zone electrophoresis, a column is filled with a single electrolyte having a specific conductivity. The mixture of substances to be separated is applied as a narrow band to the head of a buffer filled column in a band whose width is much less than the length of the column and at a concentration too low to affect the buffer conductivity. An electric field is then applied across the length of the column and the individual substances migrate and separate according to their net electrophoretic velocities. Zone electrophoresis carried out in small diameter (<100 ..mu..m) fused silica capillaries is a relatively new approach to the high resolution separation of aqueous samples. Very small volume samples (picoliter range) with separation efficiencies on the order of 10/sup 6/ theoretical plates for amino acids have been achieved. The method can be further enhanced by the dynamic combination of detection sensitivity and selectivity offered by mass spectrometry (MS). The on-line marriage of mass spectrometry to CZE is accomplished by an atmospheric pressure electrospray ionization source interface. Our research efforts have demonstratedmore » that proteins with MW's greater than 100 kDa can be analyzed using a conventional quadrupole mass spectrometer with an upper m/z limit of only 1700. 6 refs.« less

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
OSTI Identifier:
5911658
Report Number(s):
PNL-SA-16622; CONF-8905166-3
ON: DE89014466
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: American Society for Mass Spectroscopy annual symposium, Miami Beach, FL, USA, 22 May 1989
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; DETECTION; OPTIMIZATION; SEPARATION EQUIPMENT; EVALUATION; ELECTROPHORESIS; MASS SPECTROSCOPY; PEPTIDES; PROTEINS; SEPARATION PROCESSES; TRACE AMOUNTS; ORGANIC COMPOUNDS; SPECTROSCOPY; 440300* - Miscellaneous Instruments- (-1989); 550200 - Biochemistry

Citation Formats

Loo, J. A., Udseth, H. R., and Smith, R. D. Capillary zone electrophoresis-mass spectrometry of peptides and proteins. United States: N. p., 1989. Web.
Loo, J. A., Udseth, H. R., & Smith, R. D. Capillary zone electrophoresis-mass spectrometry of peptides and proteins. United States.
Loo, J. A., Udseth, H. R., and Smith, R. D. Mon . "Capillary zone electrophoresis-mass spectrometry of peptides and proteins". United States. https://www.osti.gov/servlets/purl/5911658.
@article{osti_5911658,
title = {Capillary zone electrophoresis-mass spectrometry of peptides and proteins},
author = {Loo, J. A. and Udseth, H. R. and Smith, R. D.},
abstractNote = {Capillary zone electrophoresis (CZE) is attracting extensive attention as a fast, high resolution analytical and micro-preparative separations technique for systems of biological interest. In zone electrophoresis, a column is filled with a single electrolyte having a specific conductivity. The mixture of substances to be separated is applied as a narrow band to the head of a buffer filled column in a band whose width is much less than the length of the column and at a concentration too low to affect the buffer conductivity. An electric field is then applied across the length of the column and the individual substances migrate and separate according to their net electrophoretic velocities. Zone electrophoresis carried out in small diameter (<100 ..mu..m) fused silica capillaries is a relatively new approach to the high resolution separation of aqueous samples. Very small volume samples (picoliter range) with separation efficiencies on the order of 10/sup 6/ theoretical plates for amino acids have been achieved. The method can be further enhanced by the dynamic combination of detection sensitivity and selectivity offered by mass spectrometry (MS). The on-line marriage of mass spectrometry to CZE is accomplished by an atmospheric pressure electrospray ionization source interface. Our research efforts have demonstrated that proteins with MW's greater than 100 kDa can be analyzed using a conventional quadrupole mass spectrometer with an upper m/z limit of only 1700. 6 refs.},
doi = {},
url = {https://www.osti.gov/biblio/5911658}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1989},
month = {5}
}

Conference:
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