Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein

Journal Article · Fri May 01 04:00:00 EDT 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.5.2717· OSTI ID:5896362

Benyajati, C; Place, A R; Powers, D A; Sofer, W

The gene that codes for Drosophila alcohol dehydrogenase was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid sequence of the ADH. Two intervening DNA sequences (introns) were identified within the protein encoding region. Both contained the 5' G-T and 3' A-G dinucleotides characteristic of intron boundaries of eukaryotic genes. On the basis of secondary structure predictions, the first 140 amino acid residues of Drosophila ADH are in an alternating ..beta..-sheet/..cap alpha..-helix arrangement which is characteristic of the coenzyme binding domain of dehydrogenases. The smaller of the two introns interrupts the domain predicted to bind the adenine portion of the coenzyme.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 5896362
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:5; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACIDS
ANIMALS
ARTHROPODS
BACTERIOPHAGES
CARBOXYLIC ACIDS
DIPTERA
DNA
DROSOPHILA
ENZYMES
FLIES
FRUIT FLIES
GENETIC MAPPING
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PARASITES
VIRUSES