Organizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilis

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.6.2994· OSTI ID:5145038

Yao, Kwokming; White, K ^[1]

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1. Brandeis Univ., Waltham, MA (United States)

Drosophila virilis genomic DNA corresponding analysis of a 3.8-kb genomic piece allowed identification of (1) an open reading frame (ORF) with striking homology to the previously identified D. melanogaster ORF and (2) conserved sequence elements of possible regulatory relevance within and flanking the second intron. Conceptual translation of the D. virilis ORF predicts a 519-amino-acid-long ribonucleoprotein consensus sequence-type protein. Similar to D. melanogaster ELAV protein, it contains three tandem RNA-binding domains and an alanine/glutamine-rich amino-terminal region. The sequence throughout the RNA-binding domains, comprising the carboxy-terminal 346 amino acids, shows an extraordinary 100% identify at the amino acid level, indicating a strong structural constraint for this functional domain. Thus, the divergence of the amino-terminal region of the ELAV protein reflects lowered functional constraint rather than species-specific functional specification.

• OSTI ID: 5145038
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:6; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ARTHROPODS
BIOLOGICAL EVOLUTION
CARBOXYLIC ACIDS
DIPTERA
DNA SEQUENCING
DROSOPHILA
FLIES
FRUIT FLIES
GENES
GLUTAMIC ACID
INSECTS
INVERTEBRATES
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS