Biological effects and irradiation dose induced in human lymphocytes in vitro by an intracellular radionuclide: /sup 99m/Tc

Journal Article · Sun May 01 04:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575962· OSTI ID:5895944

Meignan, M ^[1]; Charpentier, B; Wirquin, E; Chavaudra, J; Fries, D; Galle, P

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1. Faculte de Medecine, Creteil, France

Since lymphocyte radiolabeling is widely used for in vitro and in vivo studies and may interfere with the properties of the cells, the purpose of this work was to investigate the effects of /sup 99m/Tc labeling on lymphocyte morphology and functions. Two ranges of /sup 99m/Tc activity were used, 37 and 370 MBq, and their effects were compared to those produced by a graduated external irradiation. Electron microscopy was performed and T cells were studied using biological markers (E-Rosette formation, cell proliferation under lectin reactivity, and specific cell-mediated cytolysis). After 370-MBq labeling, an intense cytotoxic effect was observed on cell morphology, drastically impeding T-lymphocyte functions. Thirty-seven-megabecquerel labeling and sham procedure did not significantly affect cell functions. The external irradiation dose-response curve indicated a clear radiation-induced inhibitory effect, especially over 10 Gy. This effect was less intense than that obtained with 370-MBq /sup 99m/Tc labeling. The labeling cytotoxic effect can then be attributed to a radiolesion due to a radiation dose which is probably higher than 10 Gy. In fact, the dose received by lymphocytes during the 370-MBq labeling procedure was estimated as 70 Gy by calculations, taking into account all emissions of /sup 99m/Tc.

• OSTI ID: 5895944
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 94:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTRON MICROSCOPY
HOURS LIVING RADIOISOTOPES
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIATION DOSES
RADIATION EFFECTS
RADIOISOTOPES
SOMATIC CELLS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TOXICITY
TRANSMISSION ELECTRON MICROSCOPY
VERTEBRATES
YEARS LIVING RADIOISOTOPES