Rapid electrothermal atomic absorption spectrophotometric method for cadmium and lead in human whole blood

Journal Article · Sun Nov 01 04:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5889545

Subramanian, K S; Meranger, J C

A rapid graphite-furnace atomic absorption spectrophotometric procedure is described for determining cadmium and lead in heparinized human whole blood. A known aliquot of the blood sample is diluted fivefold with an aqueous solution composed of 5 g each of diammonium hydrogen phosphate and Triton X-100 per liter, the solution is vigorously agitated, and a 10-..mu..L aliquot is injected into a pyrocoated graphite tube under optimized instrumental conditions. Values for Cd and Pb in the sample are obtained by direct comparison to linear working curves prepared from aqueous standards of the metals in the diammonium hydrogen phosphate-Triton medium; there is no need to use the method of standard addition or matrix-matched calibration curves. Also, the method is free of matrix-matched calibration curves. Also, the method is free of matrix effects. At least 30 samples can be analyzed per hour. The rapidity, simplicity, and sensitivity of the method make it attractive as a screening technique for routine environmental surveillance involving large throughput of samples.

• Research Organization: Health and Welfare Canada, Ottawa
• OSTI ID: 5889545
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:11; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
APPROPRIATE TECHNOLOGY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CADMIUM
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
DATA
ELEMENTS
EXPERIMENTAL DATA
INFORMATION
LEAD
MATERIALS
MEASURING METHODS
METALS
NUMERICAL DATA
QUANTITATIVE CHEMICAL ANALYSIS
SCREENING
SENSITIVITY
SPECTROPHOTOMETRY