A micromethod for the determination of blood lead with matrix modification by graphite furnace atomic absorption utilizing a L`VOV platform

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:191750

Sheier, D R ^[1]

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1. Bellin Hospital, Green Bay, WI (United States)

The effect of low concentrations of lead on pre- and post-natal growth and development is a growing concern. Several hundred thousand children, most of them living in older houses, are at risk of ingesting lead-based paint as well as lead bearing soil and house dust contaminated by the deterioration of lead-based paint. The authors have developed a micromethod utilizing 40 microliters of whole blood for the determination of lead in whole blood. Platform furnace technology coupled with the use of a matrix modifier has enabled the determination of lead in whole blood using aqueous standards for creating the calibration curve. The matrix modifier is prepared with 0.2% Triton X-100, 0.5% Ammonium Phosphate Dibasic, and 0.2% Nitric Acid. The standards are prepared in 1% Nitric Acid and diluted 1:10 with matrix modifier. The aqueous standards are compared to the NIST Standard Reference Material 955a and the values are adjusted accordingly. The controls and patient samples are prepared by diluting 40 microliters of whole blood with 360 microliters of matrix modifier. The sample is dried in two steps (120{degrees} and 250{degrees} C), charred at 615{degrees} C, and atomized at 1700{degrees} C. The 283.3 nm lead line is used because the background absorbance is lower and the calibration curve more linear. Blood controls are obtained from BioRad and show the following CV`s: Level 1 (7.8 ug/dl) 12.4; Level 2 (23.1 ug/dl) 5.3; and Level 3 (54.6 ug/dl) 4.2. The sensitivity of the method is 1 ug/dl. The recovery of whole blood spiked with 10 ug/dl of lead standard is 101%. The quality control data and recovery studies indicate that this method is very good for the determination of lead in whole blood.

• OSTI ID: 191750
• Report Number(s): CONF-941098--
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ABSORPTION SPECTROSCOPY
BLOOD
CALIBRATION
CHEMICAL ANALYSIS
LEAD
SAMPLE PREPARATION