Identification of a novel human RAD51 homolog, RAD51B

Journal Article · Mon Dec 15 04:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1997.5062· OSTI ID:588659

Albala, J S; Thelen, M P; Prange, C ^[1]

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1. Lawrence Livermore National Lab., CA (United States); and others

The highly conserved Saccharomyces cerevisiae RAD51 protein functions in both mitotic and meiotic homologous recombination and in double-strand break repair. Screening of the public cDNA sequence database for RAD51-like genes led to the identification of a partial sequence from a breast tissue library present in the I.M.A.G.E. (Integrated Molecular Analysis of Genes and their Expression) collection. An extended 1764-bp cDNA clone encoding an open reading frame of 350 amino acids was isolated. This clone showed significant amino acid identity with other human RAD51 homologs. The new homolog, named RAD51B, was mapped to human chromosome 14q23-q24.2 using a panel of human-hamster somatic cell hybrids and fluorescence in situ hybridization. Northern blot analysis demonstrated that RAD51B mRNA is widely expressed and most abundant in tissues active in recombination. Functions associated with known RAD51 homologs suggest a role for RAD51B in meiotic recombination and/or recombinational repair. 30 refs., 2 figs.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 588659
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 46; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
CELL CYCLE
DNA
DNA REPAIR
DNA SEQUENCING
FLUORESCENCE
GENE RECOMBINATION
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 14
IN-SITU HYBRIDIZATION
PHENOTYPE
PROTEINS
RADIOSENSITIVITY
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION