Nuclear localization of Rad51B is independent of BRCA2
Human Rad51 is critical for the maintenance of genome stability through its role in the repair of DNA double-strand breaks. Rad51B (Rad51L1/hRec2) is one of the five known paralogs of human Rad51 found in a multi-protein complex with three other Rad51 paralogs, Rad51C, Rad51D and Xrcc2. Examination of EGFP-Rad51B fusion protein in HeLa S3 cells and immunofluorescence in several human cell lines confirms the nuclear localization of Rad51B. This is the first report to detail putative interactions of a Rad51 paralog protein with BRCA2. Utilization of a BRCA2 mutant cell line, CAPAN-1 suggests that Rad51B localizes to the nucleus independent of BRCA2. Although both Rad51B and BRCA2 are clearly involved in the homologous recombinational repair pathway, Rad51B and BRCA2 do not appear to associate directly. Furthermore, mutations in the KKLK motif of Rad51B, amino acid residues 4-7, mislocalizes Rad51B to the cytoplasm suggesting that this is the nuclear localization signal for the Rad51B protein. Examination of wild-type EGFP-Rad51B fusion protein in mammalian cells deficient in Rad51C showed that Rad51B localizes to the nucleus independent of Rad51C; further suggesting that Rad51B, like Rad51C, contains its own nuclear localization signal.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 877911
- Report Number(s):
- UCRL-JRNL-213335
- Journal Information:
- Mutagenesis, Journal Name: Mutagenesis Journal Issue: 1 Vol. 20; ISSN MUTAEX; ISSN 0267-8357
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rad51C deficiency destabilizes XRCC3, impairs recombination and radiosensitizes S/G2-phase cells
Disparate requirements for the Walker A and B ATPase motifs ofhuman RAD51D in homologous recombination
Differential roles of XRCC2 in S-phase RAD51 focus formation induced by DNA replication inhibitors
Journal Article
·
Sat May 01 00:00:00 EDT 2004
· Journal of Biological Chemistry
·
OSTI ID:838067
Disparate requirements for the Walker A and B ATPase motifs ofhuman RAD51D in homologous recombination
Journal Article
·
Fri Apr 21 00:00:00 EDT 2006
· Nucleic Acids Research
·
OSTI ID:901820
Differential roles of XRCC2 in S-phase RAD51 focus formation induced by DNA replication inhibitors
Journal Article
·
Fri May 14 00:00:00 EDT 2004
· Journal of Biological Chemistry
·
OSTI ID:15020070