Direct and indirect polarimetry for detection in microbore liquid chromatography

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00273a009· OSTI ID:5880579

Bobbitt, D R; Yeung, E S

An optical activity detection system is described that is compatible with microbore LC (liquid chromatography). The scope of the detection scheme can be expanded to include optically inactive materials when an optically active eluent is used. In addition, with chromatograms of an analyte in two different eluents, one optically active and the other the racemic analog of the first, the volume fraction of the analyte can be determined without the need for identification or standards. A detectability of 11 ng is obtained for fructose.

• Research Organization: Iowa State Univ., Ames
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5880579
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 56:9; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
CARBOHYDRATES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
FRUCTOSE
HEXOSES
INFORMATION
KETONES
LIQUID COLUMN CHROMATOGRAPHY
MONOSACCHARIDES
NUMERICAL DATA
OPTICAL ACTIVITY
ORGANIC COMPOUNDS
POLARIMETERS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHARIDES
SEPARATION PROCESSES