New detection methods and techniques with applications in liquid chromatography

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5502593

Wilson, S A

Improvements in instrumental techniques for use in liquid chromatography (LC) used in detection and in the methods by which detectors are used to obtain information are reported. Results of two projects in which new detection techniques are demonstrated are described. Both of these techniques take advantage of the unique properties of lasers as light sources. The first technique is a laser-based three-dimensional detector which allows simultaneous monitoring of the refractive index, absorbance, and fluorescence from a flowing stream. Using the laser's ability to be focused to a very small spot, a one-microliter cell was built allowing compatibility with microbore column LC. The second technique is a laser-based absorbance detector which allows one to make differential absorbance measurements, optically using Michelson interferometry. A novel detection method is shown to work with two common LC detectors: the absorbance detector and the electrical conductivity detector. The absorbance detector works as a universal detector when an absorbing eluent is used, allowing one to detect nonabsorbing analytes. This mode of detection, along with the new detection scheme, is shown to allow analytes to be quantified without the need for standards. The same detection scheme is demonstrated with the conductivity detector, with nonsuppressed ion chromatography.

• Research Organization: Iowa State Univ. of Science and Technology, Ames (USA)
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5502593
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ABSORPTION
CHROMATOGRAPHY
DETECTION
FLUORESCENCE
LASERS
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
OPTICAL PROPERTIES
PHYSICAL PROPERTIES
REFRACTIVITY
SEPARATION PROCESSES