Absorption detection in microcolumn liquid chromatography via indirect polarimetry

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00279a062· OSTI ID:5896024

Bobbitt, D R; Yeung, E S

A laser-based micropolarimeter with a cell volume of 1 ..mu..gL is interfaced to microcolumn liquid chromatography. When (R)-(+)-limonene was used as the eluent for normal-phase chromatography, optically inactive analytes such as dibutyl phthalate (k'= 1.2) can be detected at the 12-ng level (S/N = 3) as an indirect polarimetric signal. When an absorbing analyte elutes, the heat generated in the detector causes a lower number density of the eluent to be present and a decrease in optical rotation is observed. This indirect absorption scheme provides a detectability of 1.8 X 10/sup -6/ AU, or 36 pg of N-methyl-o-nitroaniline injected, when 73 mW of 458-nm laser light is used. 14 references, 3 figures.

• Research Organization: Iowa State Univ., Ames
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5896024
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 57:1; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
400104 -- Spectral Procedures-- (-1987)
ABSORPTION SPECTROSCOPY
AMINES
ANILINE
AROMATICS
CARBOXYLIC ACID SALTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
INFORMATION
LASERS
LIQUID COLUMN CHROMATOGRAPHY
NUMERICAL DATA
ORGANIC COMPOUNDS
PHTHALATES
POLARIMETERS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SPECTROSCOPY