Effects of microwave exposure on the hamster immune system. III. Macrophage resistance to vesicular stomatitis virus infection
Exposure of hamsters to microwave (MW) energy (2.45 GHz, 25 mW/cm2, 1 h) resulted in activation of peritoneal macrophages (PM) to a viricidal state restricting the replication of vesicular stomatitis virus (VSV). The PM from MW-exposed hamsters were viricidal as early as 1 day after exposure and remained active for 5 days. Immunization of hamsters with vaccinia virus induced viricidal PM by 3 to 4 days and they remained active for 7 days. To test the hypothesis that thermogenic MW exposure results in the release of endotoxin across the intestinal epithelium which subsequently activates PM, hamsters were injected with lipopolysaccharide (LPS) and their viricidal activity was studied. Lipopolysaccharide in vitro (0.2 microgram) and in vivo (0.5 microgram) activated macrophages to a viricidal state. When administered in vivo, LPS (0.5 microgram) activated macrophages as early as 1 day and the activity remained for 3 days. While MW exposure of PM in vitro failed to induce viricidal activity, exposure of PM to LPS in vitro induced strong viricidal activity. This suggests that the in vivo response of PM to MW is an indirect one, which is consistent with the hypothesis that MW-induced PM viricidal activity may be mediated via LPS. In preliminary experiments, MW exposure resulted in extended survival time for hamsters challenged with a lethal dose of vesicular stomatitis virus, supporting the concept that MW-activated PM may be a useful therapeutic modality.
- Research Organization:
- Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign
- OSTI ID:
- 5877489
- Journal Information:
- Bioelectromagnetics (N.Y.); (United States), Journal Name: Bioelectromagnetics (N.Y.); (United States) Journal Issue: 4 Vol. 5:4; ISSN BLCTD
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HAMSTERS
IMMUNE REACTIONS
INFECTIVITY
MACROPHAGES
MAMMALS
MICROORGANISMS
MICROWAVE RADIATION
PARASITES
PHAGOCYTES
RADIATIONS
RETICULOENDOTHELIAL SYSTEM
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VERTEBRATES
VIRUSES