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NMR study of the effect of sugar-phosphate backbone ethylation on the stability and conformation of DNA double helix

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00387a011· OSTI ID:5876675
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Temperature variation studies of the imido proton NMR and /sup 31/P NMR resonances of the self-associated d(C-C-A-A-G-A-T-T-G-G) and d(C-C-A-A-G-p(Et)-A-T-T-G-G) duplexes (both the R and S diastereoisomers) and the heteroduplexes formed with their complementary strand d(C-C-A-A-T-C-T-T-G-G) were carried out in aqueous solution. Results demonstrate that phosphate backbone ethylation did not disrupt the interstrand hydrogen bonding involved in double-helix formation but perturbed the helix. The S isomer perturbed the duplex more than the R isomer. The line broadening patterns and faster fraying motion in the alkylated duplexes compared to those in the nonalkylated duplexes indicate that the perturbation introduced in the middle propagates along the backbone to the end of the duplex.
Research Organization:
Johns Hopkins Univ., Baltimore, MD
OSTI ID:
5876675
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:13; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKYLATION
AQUEOUS SOLUTIONS
BARYONS
CHEMICAL REACTIONS
CHEMICAL SHIFT
CONFORMATIONAL CHANGES
DISPERSIONS
DNA
ELEMENTARY PARTICLES
FERMIONS
HADRONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MIXTURES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
RESONANCE
SOLUTIONS
STABLE ISOTOPES
TEMPERATURE DEPENDENCE