Proton NMR study of the effects of the alkylated base and sugar-phosphate backbone on the structure and conformation of DNA

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5095355

Pramanik, P; Kan, L S

The modified base and/or sugar-phosphate are of prime importance for studying mutagenesis and carcinogenesis. A decadeoxynucleotide of defined sequence (structure I) was used as a model to introduce (alkylated) modifications and study their effects on the structure and conformation of the model compound by proton NMR spectroscopy. Three alkylated decadeoxynucleotides have been studied so far. Two of them are optical isomers (R and S forms) of the chiral ethylphosphotriester placed between G(5) and A(6) of (I). The third oligomer contains an 06-methyl guanine base at the G(5) position of (I). The stability and dynamic behavior of these decamers involved in a double-stranded helix (base-paired with another chemically synthesized unmodified decamer with proper sequence) are different from that of the normal oligomer with the same sequence. Additional investigation of the self-association of two decamers with the ethylphosphotriester modification also forms G-A base pairs, but with lower melting temperature, as the nonalkylated oligomer (I). Thus, the study of these modified helices may yield information on the transcriptional mechanism and rate of DNA. The possible structure of these helices will be discussed.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 5095355
• Report Number(s): CONF-8606151-; TRN: 86-034875
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
DNA
MOLECULAR STRUCTURE
HELICAL CONFIGURATION
ISOMERS
MATHEMATICAL MODELS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PROTONS
BARYONS
CONFIGURATION
ELEMENTARY PARTICLES
FERMIONS
HADRONS
MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
RESONANCE
SPECTRA
550600* - Medicine
550201 - Biochemistry- Tracer Techniques