Physiological function of hydrogen metabolism during growth of sulfidogenic bacteria on organic substrates

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · J. Bacteriol.; (United States)

OSTI ID:5874468

Lupton, F S; Conrad, R; Zeikus, J G

Desulfovibrio vulgaris Madison and Thermodesulfobacterium commune contained functionally distinct hydrogenase activities, one which exchanged /sup 3/H/sub 2/ into /sup 3/H/sub 2/O and was inhibited by carbon monoxide and a second activity which produced H/sub 2/ in the presence of CO. Cell suspensions of D. vulgaris used either lactate, pyruvate, or CO as the electron donor for H/sub 2/ production in the absence of sulfate. Both sulfidogenic species produced and consumed hydrogen as a trace gas during growth on lactate or pyruvate as electron donors and on thiosulfate or sulfate as electron acceptors. Higher initial levels of hydrogen were detected during growth on lactate-sulfate than on pyruvate-sulfate. D. vulgaris but not T. commune also produced and then consumed CO during growth on organic electron donors and sulfate or thiosulfate. High partial pressures of exogenous H/sub 2/inhibited growth and substrate consumption when D. vulgaris was cultured on pyruvate alone but not when it was metabolizing pyruvate plus sulfate or lactate plus sulfate. The data are disucssed in relation to supporting two different models for the physiological function of H/sub 2/ metabolism during growth of sulfidogenic bacteria on organic electron donors plus sulfate. A trace H/sub 2/ transformation model is proposed for control of redox processes during growth on either pyruvate or lactate plus sulfate, and an obligate H/sub 2/ cycling model is proposed for chemiosmotic energy coupling during growth on CO plus sulfate. 29 references, 8 figures.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5874468
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 159:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BINDING ENERGY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
DESULFOVIBRIO
ELECTRONS
ELEMENTARY PARTICLES
ELEMENTS
ENERGY
ENZYME ACTIVITY
ENZYMES
FERMIONS
GROWTH
HYDROGEN
HYDROGENASES
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
LEPTONS
MATHEMATICAL MODELS
METABOLISM
MICROORGANISMS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PYRUVIC ACID
REDOX POTENTIAL
SULFATE-REDUCING BACTERIA
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS