Global transcriptomics analysis of the Desulfovibrio vulgaris change from syntrophic growth with Methanosarcina barkeri to sulfidogenic metabolism
Abstract Desulfovibrio vulgaris is a metabolically flexible microorganism. It can use sulfate as electron acceptor to catabolize a variety of substrates, or in the absence of sulfate can utilize organic acids and alcohols by forming a syntrophic association with hydrogen scavenging partner to relieve inhibition by hydrogen. These alternativemetabolic types increase the chance of survival for D. vulgaris in environments where one of the potential external electron acceptors becomes depleted. In this work, whole-genome D. vulgaris microarrays were used to determine relative transcript levels as D. vulgaris shifted its metabolism from syntroph in a lactate-oxidizing dual-culture with Methanosarcina barkeri to a sulfidogenic metabolism. Syntrophic dual-cultures were grown in two independent chemostats and perturbation was introduced after six volume changes with the addition of sulfate. The results showed that 132 genes were differentially expressed in D. vulgaris 2 hours after addition of sulfate. Functional analyses suggested that genes involved in cell envelope and energy metabolism were the most regulated when comparing syntrophic and sulfidogenic metabolism. Up-regulation was observed for genes encoding ATPase and the membrane-integrated energy conserving hydrogenase (Ech) when cells shifted to a sulfidogenic metabolism. A five-gene cluster encoding several lipo- and membrane-bound proteins was down-regulated when cells were shifted to a sulfidogenic metabolism. Interestingly, this gene cluster has orthologs found only in another syntrophic bacterium Syntrophobacter fumaroxidans and four recently sequenced Desulfovibrio strains. This study also identified several novel c-type cytochrome encoding genes which may be involved in the sulfidogenic metabolism.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1000605
- Report Number(s):
- PNNL-SA-73743; ISSN 1465-2080; MROBEO; TRN: US201101%%407
- Journal Information:
- Microbiology, 156(9):2746-2756, Vol. 156, Issue 9; ISSN 1350-0872
- Country of Publication:
- United States
- Language:
- English
Similar Records
Development and Assessment of Whole-Genome Oligonucleotide Microarrays to Analyze an Anaerobic Microbial Community and its Responses to Oxidative Stress
Anaerobic degradation of lactate by syntrophic associations of methanosarcina barkeri and desulfovibrio species and effect of H2 on acetate degradation
Related Subjects
ALCOHOLS
BINDING ENERGY
CYTOCHROMES
DESULFOVIBRIO
ELECTRONS
FUNCTIONALS
GENES
HYDROGEN
HYDROGENASES
METABOLISM
ORGANIC ACIDS
PROTEINS
SCAVENGING
STRAINS
SUBSTRATES
SULFATES
VALENCE
SULFATE-REDUCING BACTERIA
GENE-EXPRESSION ANALYSIS
ELECTRON-TRANSFER
METHANE PRODUCTION
HEAT-SHOCK
OLIGONUCLEOTIDE MICROARRAYS
VULGATIS HILDENBOROUGH
ANAEROBIC-BACTERIA
MASS-SPECTROMETRY
OXIDATIVE STRESS