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Global transcriptomics analysis of the Desulfovibrio vulgaris change from syntrophic growth with Methanosarcina barkeri to sulfidogenic metabolism

Journal Article · · Microbiology, 156(9):2746-2756
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Abstract Desulfovibrio vulgaris is a metabolically flexible microorganism. It can use sulfate as electron acceptor to catabolize a variety of substrates, or in the absence of sulfate can utilize organic acids and alcohols by forming a syntrophic association with hydrogen scavenging partner to relieve inhibition by hydrogen. These alternativemetabolic types increase the chance of survival for D. vulgaris in environments where one of the potential external electron acceptors becomes depleted. In this work, whole-genome D. vulgaris microarrays were used to determine relative transcript levels as D. vulgaris shifted its metabolism from syntroph in a lactate-oxidizing dual-culture with Methanosarcina barkeri to a sulfidogenic metabolism. Syntrophic dual-cultures were grown in two independent chemostats and perturbation was introduced after six volume changes with the addition of sulfate. The results showed that 132 genes were differentially expressed in D. vulgaris 2 hours after addition of sulfate. Functional analyses suggested that genes involved in cell envelope and energy metabolism were the most regulated when comparing syntrophic and sulfidogenic metabolism. Up-regulation was observed for genes encoding ATPase and the membrane-integrated energy conserving hydrogenase (Ech) when cells shifted to a sulfidogenic metabolism. A five-gene cluster encoding several lipo- and membrane-bound proteins was down-regulated when cells were shifted to a sulfidogenic metabolism. Interestingly, this gene cluster has orthologs found only in another syntrophic bacterium Syntrophobacter fumaroxidans and four recently sequenced Desulfovibrio strains. This study also identified several novel c-type cytochrome encoding genes which may be involved in the sulfidogenic metabolism.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1000605
Report Number(s):
PNNL-SA-73743
Journal Information:
Microbiology, 156(9):2746-2756, Journal Name: Microbiology, 156(9):2746-2756 Journal Issue: 9 Vol. 156; ISSN MROBEO; ISSN 1465-2080; ISSN 1350-0872
Country of Publication:
United States
Language:
English

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Related Subjects

08 HYDROGEN
ALCOHOLS
ANAEROBIC-BACTERIA
BINDING ENERGY
CYTOCHROMES
DESULFOVIBRIO
ELECTRON-TRANSFER
ELECTRONS
FUNCTIONALS
GENE-EXPRESSION ANALYSIS
GENES
HEAT-SHOCK
HYDROGEN
HYDROGENASES
MASS-SPECTROMETRY
METABOLISM
METHANE PRODUCTION
OLIGONUCLEOTIDE MICROARRAYS
ORGANIC ACIDS
OXIDATIVE STRESS
PROTEINS
SCAVENGING
STRAINS
SUBSTRATES
SULFATE-REDUCING BACTERIA
SULFATES
VALENCE
VULGATIS HILDENBOROUGH