Radioimmunoscintigraphy and radioimmunotherapy with RCC MABS
The basis for immunoscintigraphy and immunotherapy depends upon selective specificity of antibody. The authors have developed multiple (A6H, C5H, D5D) reactive InGl monoclonal antibodies (MAB) utilizing surgical tissue homogenates and renal cell carcinoma (RCC) cell lines. Selective specificity was demonstrated by cell binding ELISA and in vitro immunohistochemistry. MABS were labeled with I-13l (s.a. 10 ..mu..Ci/..mu..g) and RCC xenografts were imaged in 160 nude mice. Specificity index (SI) (tissue/blood cpm/g) was calculated for normal tissue, control xenograft and RCC xengograft and ranged 5-25 for RCC and <1 for normal tissue. RCC xenografts imaged at 5, 24 48 hours without background subtraction demonstrated tumors as small as 40 mg. Tl evaluate effectiveness of radifoimmunotherapy, mice bearing RCC xenografts received: no treatment, non-radiolabeled A6H, I-131-labeled control IgGl MAB (I-131-AFP22) 100 ..mu..Ci), I-131-labeled A6H (100 ..mu..Ci). Mice receiving no treatment, non-labeled A6H or labeled control MAB exhibited rapid progression of disease, while mice treated with I-131-labeled A6H exhibited 50% reduction in tumor size at 60 days. Histologic examination of tissue revealed fibrosis and significant reduction of tumor cells. Dosimetry indicated labeled A6H resulted in total body dose of 300 rads and RCC xenograft of 5,500 rads. The authors conclude that preselected RCC MABS localize in target tissue sufficient for imaging without background subtraction or extensive computer processing. In addition, effective radioimmunotherapy can be demonstrated in RCC xenografts with significant arrest of growth and reduction in tumor size.
- Research Organization:
- V.A. Medical Center, Minneapolis, MN
- OSTI ID:
- 5872231
- Report Number(s):
- CONF-850611-
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DRUGS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MEDICINE
METABOLIC DISEASES
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOIMMUNOLOGY
RADIOIMMUNOSCINTIGRAPHY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RADIOPHARMACEUTICALS
RADIOTHERAPY
RODENTS
SCINTISCANNING
THERAPEUTIC USES
THERAPY
TUMOR CELLS
USES
VERTEBRATES