Widespread expression of BDNF but not NT3 by target areas of basal forebrain cholinergic neurons

Journal Article · Fri Oct 12 00:00:00 EDT 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.1688328· OSTI ID:5871624

Phillips, H S; Hains, J M; Laramee, G R; Rosenthal, A; Winslow, J W ^[1]

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1. Genentech, San Francisco, CA (USA)

Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) are homologs of the well-known neurotrophic factor nerve growth factor. The three members of this family display distinct patterns of target specificity. To examine the distribution in brain of messenger RNA for these molecules, in situ hybridization was performed. Cells hybridizing intensely to antisense BDNF probe were located throughout the major targets of the rat basal forebrain cholinergic system, that is, the hippocampus, amygdala, and neocortex. Strongly hybridizing cells were also observed in structures associated with the olfactory system. The distribution of NT3 mRNA in forebrain was much more limited. Within the hippocampus, labeled cells were restricted to CA2, the most medial portion of CA1, and the dentate gyrus. In human hippocampus, cells expressing BDNF and mRNA are distributed in a fashion similar to that observed in the rat. These findings point to both basal forebrain cholinergic cells and olfactory pathways as potential central targets for BDNF.

• OSTI ID: 5871624
• Journal Information: Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 250:4978; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
COMPUTERIZED SIMULATION
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DNA HYBRIDIZATION
DRUGS
ESTERS
EVEN-ODD NUCLEI
GROWTH FACTORS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MITOGENS
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PRIMATES
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RATS
RNA
RODENTS
SIMULATION
SOMATIC CELLS
SPECIFICITY
SULFUR 35
SULFUR ISOTOPES
TISSUE DISTRIBUTION
VERTEBRATES