Localization of insulin receptor mRNA in rat brain by in situ hybridization
- Veterans Affairs Medical Center, Seattle, WA (USA)
Insulin receptor mRNA was demonstrated in rat brain slices by in situ hybridization with three {sup 35}S-oligonucleotide probes and contact film autoradiography. Specificity was confirmed by showing that (a) excess unlabeled probe abolished the signal, (b) an oligonucleotide probe for rat neuropeptide Y mRNA showed a different distribution of hybridization signal, and (c) the distribution of insulin receptor binding was consistent with the distribution of insulin receptor mRNA. Insulin receptor mRNA was most abundant in the granule cell layers of the olfactory bulb, cerebellum and dentate gyrus, in the pyramidal cell body layers of the pyriform cortex and hippocampus, in the choroid plexus and in the arcuate nucleus of the hypothalamus.
- OSTI ID:
- 6324369
- Journal Information:
- Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 127:6; ISSN ENDOA; ISSN 0013-7227
- Country of Publication:
- United States
- Language:
- English
Similar Records
Localization of the mRNA for the dopamine D sub 2 receptor in the rat brain by in situ hybridization histochemistry
Systemic injection of kainic acid: Gliosis in olfactory and limbic brain regions quantified with ( sup 3 H)PK 11195 binding autoradiography
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HORMONES
INSULIN
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MESSENGER-RNA
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
OLFACTORY BULBS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RNA
RODENTS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES