Molecular cloning of a human gene that is a member of the nerve growth factor family
- Howard Hughes Medical Institute, San Francisco, CA (USA)
Cell death within the developing vertebrate nervous system is regulated in part by interactions between neurons and their innervation targets that are mediated by neurotrophic factors. These factors also appear to have a role in the maintenance of the adult nervous system. Two neurotrophic factors, nerve growth factor and brain-derived neurotrophic factor, share substantial amino acid sequence identity. The authors have used a screen that combines polymerase chain reaction amplification of genomic DNA and low-stringency hybridization with degenerate oligonucleotides to isolate human BDNF and a human gene, neurotrophin-3, that is closely related to both nerve growth factor and brain-derived neurotrophic factor. mRNA products of the brain-derived neurotrophic factor and neurotrophin-3 genes were detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. Neurotrophin-3 is also expected to function in embryonic neural development.
- OSTI ID:
- 5584538
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:20; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GROWTH FACTORS
HYBRIDIZATION
MAMMALS
MAN
MESSENGER-RNA
MITOGENS
MOLECULAR STRUCTURE
NERVES
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES