Effects of the in vitro administered ethanol and lipopolysaccharide toxin on membrane properties, intracellular free calcium and phagocytic function of isolated rat kupffer cells
- Univ. of Arizona, Tucson (United States)
Low concentrations of ethanol slightly stimulated phagocytosis of cultured Kupffer cells (KC), producing practically no effect on membrane microviscosity and cytosolic free (Ca{sup 2+}){sub i}. On the contrary, high concentrations of ethanol significantly suppressed phagocytic function, increased fluidity of membrane lipids and caused a sustained rise in (Ca{sup 2}){sub i}; above the resting level of 41-85 nM. Treatment of KC with colchicine and cytochalasin B dramatically destructurized the plasma membrane lipids. Short term preincubation of KC with high doses of alcohol stimulated the disordering effects of both drugs, suggesting direct interaction of ethanol with microtubule and microfilament structures. The authors hypothesize that ethanol impairs phagocytosis of KC by concerted actions on membrane lipid fluidity, cytosolic free Ca{sup 2+} and functioning of cytoskeleton. On the other hand, incubation of KC with low concentrations of lipopolysaccharide (LPS) produced no changes in (Ca{sup 2+}){sub i}; or plasma membrane fluidity but reduced by several fold the fluidizing effect of subsequently added ethanol. They suggested that low doses of LPS, by activating second messengers other than Ca{sup 2+}, alter the functioning of the cytoskeleton and cause reorganization of the plasma membrane thus making KC membranes more resistent to the fluidizing action of ethanol and partially restoring the phagocytic function.
- OSTI ID:
- 5863650
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
ETHANOL
HYDROXY COMPOUNDS
IN VITRO
LIPIDS
LIPOPOLYSACCHARIDES
MACROPHAGES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METABOLISM
ORGANIC COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
PHYSICAL PROPERTIES
POLYSACCHARIDES
PROTEINS
RATS
RODENTS
SACCHARIDES
SECRETION
SOMATIC CELLS
VERTEBRATES