Asbestos effects on superoxide production: an in vitro study of hamster alveolar macrophages

Journal Article · Tue Apr 01 00:00:00 EST 1986 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(86)80056-1· OSTI ID:5859072

Case, B W; Ip, M P.C.; Padilla, M; Kleinerman, J

Inhaled asbestos induces accumulation of alveolar macrophages (AM) and polymorphonuclear leukocytes (PMN) in lung. Asbestos-enhanced production of superoxide anion (O-/sub 2/) by AM and/or PMN may be involved in the pathogenesis of asbestos-induced fibrosis, either through direct effects on collagen synthesis or via mediation of tissue injury and repair. In in vivo experiments, bronchoalveolar lavage (BAL) 3 to 8 weeks following intratracheal asbestos infections showed increases in both PMN and AM, with AM representing 78 to 82% of cells recovered. Inhalation models, generally regarded as more analogous to human exposures, have confirmed AM as the predominant component of the cellular response to inhaled asbestos. In this study, the in vitro effects of asbestos fiber on O-/sub 2//sup -/ production by AM have been determined in cell populations derived from the Syrian golden hamster. AM for in vitro study were obtained by BAL. O-/sub 2//sup -/ production was monitored as superoxide dismutase (SOD) - inhibitable cytochrome c reduction. Significant rises in O-/sub 2//sup -/ release by AM were noted in the presence of 0.4 mg/ml crocidolite. Chrysotile induced levels of O-/sub 2//sup -/ release in AM which were similar to those evoked by crocidolite.

• Research Organization: Mount Sinai School of Medicine, New York, NY
• OSTI ID: 5859072
• Journal Information: Environ. Res.; (United States), Vol. 39:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ASBESTOS
TOXICITY
MACROPHAGES
CELL PROLIFERATION
SUPEROXIDE RADICALS
BIOCHEMICAL REACTION KINETICS
ANIMALS
COMPARATIVE EVALUATIONS
CYTOCHROMES
FIBERS
FIBROSIS
HAMSTERS
IN VITRO
INHALATION
LEUKOCYTES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
INTAKE
KINETICS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHAGOCYTES
PIGMENTS
PROTEINS
RADICALS
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)