Transport and intracellular distribution of copper in a human hepatoblastoma cell line, HepG2
Journal Article
·
· Hepatol.; (United States)
The uptake of radiocopper by HepG2 cells is a saturable, temperature-dependent and cellular energy-independent process with a Vmax of 7.1 +/- 0.2 pmoles min-1 mg protein-1 and an estimated Km of 3.3 +/- 0.5 microM. The rate of copper uptake is reduced at an equimolar concentration of albumin and is unaffected by zinc at a 10-fold molar excess. Approximately 70% of the newly incorporated radiocopper binds to membranes and organelles, while 30% is recovered in the cytosol. The soluble fraction can be resolved into two copper-binding protein peaks. Incubation of HepG2 with nonisotopic copper results in displacement of radiocopper associated with the proteins contained in the lower molecular weight peak. Exposure of the cells to cycloheximide inhibits the incorporation of the isotope into this fraction.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY
- OSTI ID:
- 5858907
- Journal Information:
- Hepatol.; (United States), Journal Name: Hepatol.; (United States) Vol. 1; ISSN HPTLD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560171 -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Man-- (-1987)
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
COPPER
COPPER ISOTOPES
ELEMENTS
IMIDES
INHIBITION
ISOTOPES
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
TRANSITION ELEMENTS
TUMOR CELLS
UPTAKE
ZINC
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
COPPER
COPPER ISOTOPES
ELEMENTS
IMIDES
INHIBITION
ISOTOPES
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
TRANSITION ELEMENTS
TUMOR CELLS
UPTAKE
ZINC