Copper transport: Insights into a ceruloplasmin-based delivery system
Journal Article
·
· Advances in Experimental Medicine and Biology; (USA)
- Texas A M Univ., College Station (USA)
Ceruloplasmin binds to the membranes of K562 cells. The binding has been shown to result in a temperature-dependent transfer of ceruloplasmin-bound copper into the cytosol. Ascorbic acid (100 microM) stimulates the transmembrane transfer nearly 10-fold, depending on the initial concentration of {sup 67}Cu-ceruloplasmin. The protein moiety of ceruloplasmin does not enter the cells. Bathocuproine disulfonate, a chelator specific for cuprous copper, inhibits the uptake, suggesting copper atoms are reduced concomitant with their removal from ceruloplasmin. Cytosolic {sup 67}Cu from ceruloplasmin was found mainly bound to Cu, Zn superoxide dismutase, the major cytosolic copper protein in these cells. Evidence supporting the various phases in the ceruloplasmin-mediated transport mechanism are presented.
- OSTI ID:
- 6855229
- Journal Information:
- Advances in Experimental Medicine and Biology; (USA), Journal Name: Advances in Experimental Medicine and Biology; (USA) Vol. 258; ISSN 0065-2598; ISSN AEMBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ASCORBIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CERULOPLASMIN
COMPLEXES
COPPER
COPPER 67
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
GLOBULINS
GLOBULINS-ALPHA
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE TRANSPORT
MEMBRANES
METALLOPROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SUPEROXIDE DISMUTASE
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES
VITAMINS
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ASCORBIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CERULOPLASMIN
COMPLEXES
COPPER
COPPER 67
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
GLOBULINS
GLOBULINS-ALPHA
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE TRANSPORT
MEMBRANES
METALLOPROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SUPEROXIDE DISMUTASE
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENTS
VERTEBRATES
VITAMINS